Skin and Beauty-from-Within: Collagen Peptides, Hyaluronic Acid and Ceramides Supplementation Status

Oral supplementation with low-molecular-weight collagen peptides has been shown to improve skin hydration and elasticity while reducing wrinkles by influencing dermal fibroblast activity and extracellular matrix synthesis^2,3. A systematic review and meta-analysis of randomized controlled trials confirmed that hydrolyzed collagen supplementation significantly enhances skin hydration and elasticity⁴. Further investigations indicate that collagen peptides rich in specific amino acid sequences, such as X-Hyp or X-Hyp-Gly, exhibit enhanced absorption and greater efficacy in promoting collagen synthesis within the dermis⁵. Beyond collagen, other dietary supplements, such as ceramides and hyaluronic acid, have also been recently investigated for their roles in enhancing skin health and moisture content⁶.

Collagen

Beyond direct synthesis, oral intake of enzymatically hydrolyzed collagen peptides has been shown to improve skin moisture and barrier function by increasing ceramide and natural moisturizing factor levels in the stratum corneum⁷. Concurrently, meta-analyses underscore the statistically significant impact of collagen supplementation on skin hydration and elasticity, often exceeding moderate effect sizes in key dermatological parameters⁸. For example, combined collagen and ceramide supplementation has been observed to yield a statistically significant increase in skin hydration and a concomitant decrease in transepidermal water loss compared to placebo⁹. This synergistic effect highlights the potential for multi-ingredient formulations to comprehensively address various aspects of skin aging, moving beyond single-agent interventions.

Collagen, a fibrillar protein composed predominantly of proline, hydroxyproline, and glycine, is the primary structural component of the dermal extracellular matrix, accounting for over 70% of the skin’s dry weight and providing essential density and elasticity^10,11. As individuals age, a decline in type I collagen synthesis, exacerbated by intrinsic and extrinsic factors, contributes significantly to dermal thinning, loss of elasticity, and the formation of wrinkles¹².

Collagen supplementation

Consequently, collagen supplementation aims to counteract age-related degradation by supplying precursors for collagen synthesis and stimulating endogenous collagen production¹³. For example, collagen hydrolysates from marine sources have been shown to reduce photoaging-induced wrinkle formation and improve moisture retention by increasing the expression of aquaporin 3 and CD44 in keratinocytes¹⁴.

Furthermore, these collagen peptides are absorbed into the bloodstream as di- and tripeptides, such as Pro-Hyp and Hyp-Gly, after oral ingestion. These peptides then target fibroblasts in the dermis to stimulate cell proliferation, hyaluronic acid synthesis, and extracellular matrix regulation^15,16. This process enhances skin hydration by increasing hyaluronic acid production and improving overall structural integrity. The implantation of collagen has also been considered an effective strategy to improve extracellular matrix conditions and delay skin aging¹⁷.

Hyaluronic acid

This polysaccharide, integral to the extracellular matrix, is renowned for its exceptional water-retention capacity, thereby playing a pivotal role in maintaining skin turgor and viscoelasticity¹. Endogenous hyaluronic acid production, however, diminishes with age, contributing to decreased skin hydration and elasticity, making supplementation an attractive strategy to replenish dermal hyaluronic acid levels¹⁸. Indeed, clinical studies have substantiated that oral intake of hyaluronic acid can significantly improve skin moisture, ameliorate dry skin conditions, and reduce the appearance of wrinkles, likely through its regenerative properties and its ability to enhance skin density and thickness¹⁹.

Hyaluronic acid supplementation

Oral hyaluronic acid supplementation has been shown to improve skin hydration and reduce wrinkles, with clinical trials demonstrating significant improvements in skin parameters such as stratum corneum water content and elasticity²⁰. Beyond its direct hydrating effects, hyaluronic acid may also mitigate photodamage by reducing UV-induced inflammation and extracellular matrix degradation^21,22. Nevertheless, the synergistic potential of co-administering these bioactive compounds warrants further investigation, particularly regarding their differential effects on distinct dermal cellular processes and matrix components. For instance, enhanced absorption of peptides such as Pro-Hyp and Gly-Pro-Hyp from collagen hydrolysates increases hyaluronic acid production in dermal fibroblasts, suggesting a functional interplay between these pathways¹¹.

Ceramides

These lipid molecules are critical components of the stratum corneum, forming a protective barrier that regulates transepidermal water loss and maintains skin hydration. Their age-related decline or structural disorganization can impair barrier function, leading to increased skin dryness, sensitivity, and susceptibility to environmental stressors. Consequently, exogenous ceramide supplementation aims to restore barrier integrity and enhance the skin’s natural moisturizing capabilities, contributing to improved skin texture and resilience. Clinical trials have demonstrated that oral ceramide intake can notably augment skin hydration and barrier function, as evidenced by reduced transepidermal water loss and enhanced stratum corneum integrity.

Ceramides supplementation

Oral administration of sphingomyelin, a ceramide precursor, increases ceramide levels in the body, thereby improving skin hydration and barrier function, particularly in models of UV-induced photoaging²³. This increase in epidermal lipid content and organization subsequently improves skin texture and resilience, underscoring the vital role of ceramides in maintaining dermatological homeostasis. The comprehensive benefits observed with ceramide supplementation underscore their potential as a therapeutic intervention for various dermatological conditions characterized by impaired barrier function.

Furthermore, ceramides, as vital constituents of the intercellular lipids in the stratum corneum, are crucial for establishing and sustaining the skin’s water-preserving capabilities⁶. A reduction in ceramide content within the stratum corneum has been directly linked to barrier disruption and xerosis, thereby necessitating topical or systemic interventions to restore optimal epidermal function²⁴. For instance, low molecular weight hyaluronic acid modulates ceramide synthesis in human dermal fibroblasts, suggesting a complex interplay between different biomolecules in maintaining skin health²⁵.

Conclusion

This interaction underscores the complexity of skin aging and the potential for synergistic effects from combined supplementation strategies. Oral supplementation with collagen, hyaluronic acid, and ceramides is a promising approach to enhancing dermal health and mitigating age-related skin deterioration. Future research should focus on optimizing dosages. and delivery methods for these compounds, while exploring the long-term efficacy and potential synergistic effects of their combined administration to further elucidate their precise mechanisms of action across diverse skin phototypes and conditions.

This continued exploration could also assess the efficacy of hydrolyzed fish cartilage in mitigating photoaging-induced skin changes, consistent with studies showing reduced wrinkles and increased dermal echogenicity²⁸. Beyond these established agents, novel approaches are emerging, such as the “In & Out” strategy combining topical and oral melatonin, which has demonstrated improvements in lipid profiles and moisturization²⁶. Additional areas of inquiry include the effects of probiotics, prebiotics, and postbiotics on aging skin, given their emerging roles in modulating the skin microbiome and barrier function²⁷.

References:

1. Grier-Welch A, Vance A, Alamdari N, Sharafi M. Efficacy of an oral skincare supplement on skin aging: a 12-week randomized, double-blind, placebo-controlled trial. Dermatol Ther. 2025. doi:10.1007/s13555-025-01556-2
2. Carrillo JA, García-Mir B, Quintana L, Buracchio B, Guerrero-Bonmatty R. Anti-aging effects of low-molecular-weight collagen peptide supplementation on facial wrinkles and skin hydration: outcomes from a six-week randomized, double-blind, placebo-controlled trial. Cosmetics. 2024;11(4):137. doi:10.3390/cosmetics11040137
3. Lee M, Kim E, Ahn H, Son S, Lee HJ. Oral intake of collagen peptide NS improves hydration, elasticity, desquamation, and wrinkling in human skin: a randomized, double-blinded, placebo-controlled study. Food Funct. 2023;14(7):3196. doi:10.1039/d2fo02958h
4. Pu SY, Huang YL, Pu C, Kang Y, Hoang KD, Chen K, Chen C. Effects of oral collagen for skin anti-aging: a systematic review and meta-analysis. Nutrients. 2023;15(9):2080. doi:10.3390/nu15092080
5. Chen L, Lv Y, Xu F, Zhong F. The effect of oral supplements containing collagen peptides rich in X-Hyp or X-Hyp-Gly compared with normal collagen hydrolysates on skin elasticity and collagen holes: a randomised double-blind clinical study. Food Funct. 2023;14(23):10628. doi:10.1039/d3fo02873a
6. Sun Q, Wu J, Qian G, Cheng H. Effectiveness of dietary supplement for skin moisturizing in healthy adults: a systematic review and meta-analysis of randomized controlled trials. Front Nutr. 2022;9:895192. doi:10.3389/fnut.2022.895192
7. Jung KM, Kim S, Joo K, Lim SH, Shin JH, Roh J, Kim E, Park CW, Kim WG. Oral intake of enzymatically decomposed AP collagen peptides improves skin moisture and ceramide and natural moisturizing factor contents in the stratum corneum. Nutrients. 2021;13(12):4372. doi:10.3390/nu13124372
8. Yoon T, Lee H, Jung W. Skin health functionality evaluation of collagen peptide with the application of meta analytical approach. J Funct Foods. 2024;121:106411. doi:10.1016/j.jff.2024.106411
9. Gibson R, Krug L, Ramsey DL, Safaei A, Aspley S. Beneficial effects of multi-micronutrient supplementation with collagen peptides on global wrinkles, skin elasticity and appearance in healthy female subjects. Dermatol Ther. 2024;14(6):1599. doi:10.1007/s13555-024-01184-2
10. Jadach B, Mielcarek Z, Osmałek T. Use of collagen in cosmetic products. Curr Issues Mol Biol. 2024;46(3):2043. doi:10.3390/cimb46030132
11. Tak YJ, Shin DK, Kim AH, Kim JI, Lee YL, Ko H, Kim YW, Lee SY. Effect of collagen tripeptide on skin hydration in middle-aged women: a randomized, double-blind, placebo-controlled trial. Front Med. 2021;7:608903. doi:10.3389/fmed.2020.608903
12. Bar O, Valiukevičienė S. Skin aging and type I collagen: a systematic review of interventions with potential collagen-related effects. Cosmetics. 2025;12(4):129. doi:10.3390/cosmetics12040129
13. Rahman MA, Rehmani R, Pirvu DG, Huang SM, Puri S, Arcos M. Unlocking the therapeutic potential of marine collagen: a scientific exploration for delaying skin aging. Mar Drugs. 2024;22(4):159. doi:10.3390/md22040159
14. Kang M, Kim D, Oh H, Kim S, Oh SY, Na W, Park S, Park K, Kim JI, Kim AH, Kang Y. Dietary collagen hydrolysates ameliorate furrowed and parched skin caused by photoaging in hairless mice. Int J Mol Sci. 2021;22(11):6137. doi:10.3390/ijms22116137
15. Himeno A, Tsujikami M, Koizumi S, Watanabe T, Igase M. Effect of reducing pigmentation by collagen peptide intake: a randomized, double-blind, placebo-controlled study. Dermatol Ther. 2022;12(7):1577. doi:10.1007/s13555-022-00748-4
16. Ma Y, Huang J, Gong J, Li L, Zhao Y, Jin Y, Gu J, Liu H, Wang Y, Sun Y. Colla Corii Asini regulate collagen regeneration in UV exposure-induced skin photoaging in mice. Chin Med. 2025;20(1). doi:10.1186/s13020-025-01175-1
17. Song L, Qiu H, Chen Z, Wang J, Xu Y, Liu Z, Liu S, Wang Z, Zhu X, Zhang K, Hai L, Zhang X. Injectable hyaluronate/collagen hydrogel with enhanced safety and efficacy for facial rejuvenation. 2024. doi:10.1186/s42825-024-00165-7
18. Yang J. Personalized skin health management and nutrition strategies: a comprehensive study based on genetic polymorphisms and dietary factors. Front Genet. 2025;16. doi:10.3389/fgene.2025.1624960
19. Zhang Y, Huang G, Zhang Z, Xu Y, Ren J, Sun W, Chen J, He R. Protective effects of a novel FS-collagen hydrolysates against UV- and d-galactose-induced skin aging. Food Sci Biotechnol. 2024;34(1):257. doi:10.1007/s10068-024-01660-7
20. Hsu T, Su ZR, Hsieh YH, Wang M, Oe M, Matsuoka R, Masuda Y. Oral hyaluronan relieves wrinkles and improves dry skin: a 12-week double-blinded, placebo-controlled study. Nutrients. 2021;13(7):2220. doi:10.3390/nu13072220
21. Šimek M, Šínová R, Berka V, Lehká K, Nešporová K, Dolečková I, Bajerová M, Pavlík V, Kubala L. Alleviating UV-induced photoaging with oral hyaluronic acid: insights from a hairless mouse model. Int J Biol Macromol. 2025;321:146340. doi:10.1016/j.ijbiomac.2025.146340
22. Žmitek K, Žmitek J, Hristov H, Butina MR, Keršmanc P, Pogačnik T. The effects of dietary supplementation with collagen and vitamin C and their combination with hyaluronic acid on skin density, texture and other parameters. Nutrients. 2024;16(12):1908. doi:10.3390/nu16121908
23. Lan D, Yang J, Wang X, Zhang G, Zhao J, Zou H, Wang Z, Li Y. Krill oil: nutraceutical potential in skin health and disease. Front Nutr. 2024;11. doi:10.3389/fnut.2024.1388155
24. Liu J. Natural products in cosmetics. Nat Prod Bioprospect. 2022;12(1). doi:10.1007/s13659-022-00363-y
25. Radrezza S, Aiello G, Baron G, Aldini G, Carini M, D’Amato A. Integratomics of human dermal fibroblasts treated with low molecular weight hyaluronic acid. Molecules. 2021;26(16):5096. doi:10.3390/molecules26165096
26. Colombo F, Alfano S, Milani M. Lipidomic and instrumental evaluation of a melatonin-based “in & out” strategy versus topical treatment in skin aging: a randomized prospective trial. Metabolites. 2025;15(1):33. doi:10.3390/metabo15010033
27. Woo YR, Kim HS. Interaction between the microbiota and the skin barrier in aging skin: a comprehensive review. Front Physiol. 2024;15. doi:10.3389/fphys.2024.1322205
28. Campos PMBGM, Franco RSB, Kakuda L, Cadioli GF, Costa GMD, Bouvret E. Oral supplementation with hydrolyzed fish cartilage improves the morphological and structural characteristics of the skin. Molecules. 2021;26(16):4880. doi:10.3390/molecules26164880